[HTML][HTML] Role of cytosolic malate dehydrogenase in oocyte maturation and embryo development

SJ Yoon, DB Koo, JS Park, KH Choi, YM Han, KA Lee - Fertility and sterility, 2006 - Elsevier
OBJECTIVE: To elucidate the function of cytosolic malate dehydrogenase (Mor2) in oocyte
maturation and embryo development using RNA interference (RNAi). DESIGN: Experimental
animal study. SETTING: Research unit of university. ANIMAL (S): Female 4-week-old
(C57/BL6) mice. INTERVENTION (S): Isolation of immature germinal vesicle (GV) oocytes or
fertilized pronucleus (PN) embryos, microinjection of Mor2 double-stranded RNA (dsRNA),
and reverse transcription and polymerase chain reaction (RT-PCR) analysis to investigate …
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